VAHEAT

Temperature Control in High-Resolution Microscopy

Just set the temperature and start your measurement. Let VAHEAT do the rest.

Operating Temperature Range

Room temperature up to 100°C (300°C upon request). Thermally decouple your sample from the environment via local heating.

Heating Rates

Our microheater generates heating rates up to 100 K/s.

Precision

Relative temperature precision of 0.1 K. The fast thermal response allows precise temperature stabilization even when using perfusion systems.

Compatibility

Compatible with all conventional microscopes and made for live-cell imaging.

The Components

VAHEAT – The Control Unit

Thermal contact of the objective with the sample via immersion oil often causes temperature gradients within the field of view. Our specific chip design with integrated heating element and a precise temperature sensor (smart substrate) allows to overcome these problems.

  • Precisely control and measure the temperature in your sample volume
  • Define and start your individual temperature profiles
  • Set active temperature feedback
  • Send heat shocks to your sample
  • Change live the temperature via our tuning knob
  • Integrate our device in your data-acquisition software using our API

The Microscope Adapter

The size of our substrate holder is designed to fit any commercially available microscope and resembles the size of a standard microscope slide. Simply put our system onto your microscope and start measuring!

  • Footprint of a standard microscope slide (75 mm x 25 mm)
  • Compatible with nearly all common inverted and upright microscopes
  • Simple and fast change of smart substrates
  • Precise magnetic clamp mechanism
  • Ultra slim design for transmission light microscopy

Our Smart Substrates

Our substrates come with integrated and calibrated temperature sensor and microheater. We provide custom sizes and options upon request. The standard version is equipped with a small pan for your sample. Our smart substrates allow homogeneous heating in the field of view and provide precise temperature control.

  • 18 mm x 18 mm
  • 170 μm thin – ideally suited for high-resolution microscopy and live-cell imaging
  • Calibrated temperature sensor
  • Integrated microheater
  • Fast thermal response
  • Precisely and directly measure the temperature of your sample volume
  • Can be used as disposable

Thermal Problems in High-Resolution Microscopy

Uncontrolled temperature in the field-of-view

Temperature control in the field-of-view (FOV) is essential for many applications in high-resolution microscopy. Direct thermal contact of the objective with the coverslip poses fundamental problems (see images).

Conventional methods trying to solve this issue have severe drawbacks, such as limited maximum temperatures of approx. 40ºC, no fast temperature dynamics and associated long waiting times to achieve thermal equilibrium.

Our systems provides a solution to these issues.

Temperature gradients on a conventionally heated coverslip

Air objective
Oil-immersion objective

Benefits of Using VAHEAT

Set your temperature and start your measurement instantly via local heating.

Study dynamic temperature dependencies.

Heat your sample up to 100ºC with a high precision and active feedback.

Ensure a homogenous temperature in your FOV.

Our System Includes

  • Stand-alone control unit with easy to clean touch panel and USB interface

  • Substrate holder compatible with all conventional microscope stages

  • Smart substrates with integrated microheater and calibrated temperature sensor

  • Computer-Interface for real-time temperature readout and control

  • External Power-Supply

VAHEAT in Action

Thermal Management in High-Resolution Microscopy has never been this easy.

VAHEAT allows fast, dynamic and homogeneous thermal control even when using oil-immersion objectives.

VAHEAT decouples your cover slip thermally from the environment and enables fast temperature dynamics, even against strong temperature gradients.

Thermal control of n-tetracosan crystallization on the microscopic level using VAHEAT.